The ReFacto AF® Laboratory Standard was created for use with the one-stage clotting assay (or the chromogenic assay), when testing plasma samples from patients receiving ReFacto AF®.¹ It can be used exactly as you would use a normal (plasma-based) laboratory standard.²

There are 3 steps involved in preparing the ReFacto AF® Laboratory Standard. 

Step 1

• Reconstitute the ReFacto AF® Laboratory Standard at room temperature using 1.0mL of sterile water.
• After reconstitution, the vial contents should be transferred to a plastic tube with a stopper.
• The reconstituted ReFacto AF® Laboratory Standard solution should be maintained at room temperature and then used within 2 hours.*

• The Refacto AF® Laboratory Standard should be prediluted in factor VIII-deficient (haemophilic or chemically-or-immuno-depleted) plasma containing normal functional levels of von Willebrand factor to yield a 1.0IU/mL solution.
• The prepared standard should be used once and discarded.
• DO NOT REFREEZE.

• Dilute the ReFacto AF® Laboratory Standard solution in accordance with standard operating procedures to create a standard curve for analysis of plasma samples.
• Once the standard curve is established, analysis samples from patients treated with ReFacto AF® may begin.
• Discard all unused materials following universal precautions.